Mhetane measurement from ruminants

 SF6 permeation tubes in methane measurement from ruminants

 

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Methane released  by ruminant livestock  represents the largest global source of methane, with strong increase of concentration of  green house gas in atmosphere. The SF6 tracer technique is commonly  used widely for determining methane (CH4) emission rates by  ruminant animals. It is a technique available for precise and accurately determining CH4 emissions by individual grazing animals.

A permeation tube that release SF6 is placed in a cow’s rumen, the animal is fitted with a sampling system such that the air from around the mouth and nostrils can be collected over an estende period of time.

Permeation tube is a small containers in stainless steel with a small window in polymeric material filled  with pure SF6 (Sulfur Hexafluoride)  in a two-phase equilibrium between its gas phase and liquid  phase.  Whit a constant temperature, the device emits the compound through permeable wall with a constant rate. In this case the permeation tubes are certified at 39 °C (internal temperature of rumen).

The SF6 is expired together Methane product from are ruminal fermentation. The gasses are sampled into a PVC canister formed to fit around the cow’s neck. After  sampling collection period has  been completed, the canister is taken  off  the animal and connected to the dilution system and final pressure recorded. Nitrogen is then slowly added until the pressure in the canister is increased about 1,2 atm. The pressurized gasses into the canister is transfer to gas chromatograph .  Methane emission rate can be calculated with following relation:

formula ruminants

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